有效的抗支原體抗生素

為了根除FBS和胰蛋白酶的支原體，UV照射比所提及的方法更有效。與γ照射想比，UV照射不損害血清組分。紫外線照射可以消除胰蛋白酶或FBS任何潛在的污染。

需要經(jīng)常檢查以確認(rèn)支原體?是否完全根除。有時(shí)支原體被抑制并且它們的滴度降低到可檢測的水平以下。因此，采用敏感的支原體檢測手段對于探知細(xì)胞培養(yǎng)物中的任何再感染是必要的。建議在至少四至六次傳代后，重新檢查在不含抗生素的培養(yǎng)基培養(yǎng)的細(xì)胞。（需要支原體祛除試劑，請點(diǎn)擊這里！）

英文原文：

Prevention and Detection of Mycoplasma Contamination in Cell Culture

Various antibiotics with different inhibitory effects on cellular metabolism can be helpful to eliminate mycoplasma contamination. The mechanism of action of macrolides and tetracyclines is inhibiting protein synthesis, but they bind to different subunits of ribosomes. The quinolone inhibits the DNA replication by obstructing bacterial gyrase. In the case of using just one type of antibiotic, it is highly possible that mycoplasmas escape from the inhibitory mechanism or become resistant to it. Insufficient duration or concentration of antibiotic treatment can cause resistance. It is because of surviving the resistant mycoplasmas in the presence of low amount of antibiotics. These resistant mycoplasmas can neutralize the inhibitory mechanism of antibiotic or change its attack site. They can also pump the antibiotic out (18).

The efficiency of antibiotics in elimination of mycoplasmas is between 66 and 85 percent. These percentages include the cultures in which the growth of eukaryotic cells was inhibited, though. Three to 11 percent of cells which are already in a bad condition with a high infection level are lost after antibiotic treatment. However, this event depends on the antibiotic (65).

Elimination of mycoplasmas is usually difficult or unsuccessful due to the resistance of mycoplasmas to antibiotics. It is more successful to passage mycoplasma contaminated cells in nude mice; however, the recovery of cells is not always guaranteed. But when the cells can be collected from subcutaneous tumors in nude mice, the cells are free from mycoplasmas together with a large number of macrophages. Since there is no thymus in nude mice and surely no T-cell dependent immune response, it is possible the macrophages are in charge of the elimination of mycoplasmas. This hypothesis was proved by a brief co-cultivation of mycoplasma contaminated cells with mouse macrophages (75, 76).