胎牛血清胎球蛋白的鈣結(jié)合性能

　　胎球蛋白，一個(gè)豐富的蛋白質(zhì)胎牛血漿，是人類α2HS糖蛋白（α2HS）的牛同源。盡管許多研究，這兩種蛋白質(zhì)的生物學(xué)功能仍然無法實(shí)現(xiàn)。我們現(xiàn)在報(bào)告在胎牛血清胎球蛋白已印跡上電泳后的膜的顯著的45Ca提高結(jié)合活性。純化的蛋白質(zhì)的鈣結(jié)合能力通過平衡透析，這表明，牛胎球蛋白有多種鈣結(jié)合位點(diǎn)，其中之一具有0.95×10-4M的Kd值分析據(jù)還表明，胎球蛋白的鈣結(jié)合活性高于在晚期妊娠階段白蛋白在牛胎兒血清的更大。由于胎牛血清不僅含有高濃度的胎球蛋白而且鈣的高濃度，可能的是胎球蛋白功能通過鈣離子濃度的正常化保持在胎兒血清高水平鈣。

　　英文原文：

Calcium-binding properties of fetuin in fetal bovine serum        

Fetuin, an abundant protein in fetal bovine plasma, is the bovine homolog of human α2HS glycoprotein (α2HS). In spite of numerous studies, the biological functions of both proteins remain elusive. We now report the remarkable 45Ca-binding activity of fetuin in fetal bovine serum that has been blotted on a membrane after electrophoresis. The Ca-binding ability of the purified protein was analyzed by equilibrium dialysis, which revealed that bovine fetuin had multiple Ca-binding sites, one of which had a Kd of 0.95 × 10?4 M. It was also shown that the Ca-binding activity of fetuin was greater than that of albumin in serum of the bovine fetus at the late gestational stage. Since fetal bovine serum contains not only a high concentration of fetuin but also a high concentration of Ca, it is possible that fetuin functions to maintain high levels of Ca in fetal serum via normalization of the concentration of Ca2+ ions.

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